GHSR DNA hypermethylation is a common epigenetic alteration of high diagnostic value in a broad spectrum of cancers

Moskalev EA, Jandaghi P, Fallah M, Manoochehri M, Botla SK, Kolychev OV, Nikitin EA, Bubnov VV, Doeberitz MVK, Strobel O, Hackert T, Buechler MW, Giese N, Bauer A, Muley T, Warth A, Schirmacher P, Haller F, Hoheisel JD, Riazalhosseini Y (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Oncotarget Impact Journals

Book Volume: 6

Pages Range: 4418-27

Journal Issue: 6

DOI: 10.18632/oncotarget.2759

Abstract

Identification of a single molecular trait that is determinant of common malignancies may serve as a powerful diagnostic supplement to cancer type-specific markers. Here, we report a DNA methylation mark that is characteristic of seven studied malignancies, namely cancers of lung, breast, prostate, pancreas, colorectum, glioblastoma and B cell chronic lymphocytic leukaemia (CLL) (n = 137). This mark was defined by substantial hypermethylation at the promoter and first exon of growth hormone secretagouge receptor (GHSR) through bisulfite pyrosequencing. The degree of aberrant methylation was capable of accurate discrimination between cancer and control samples. The highest sensitivity and specificity of cancer detection was achieved for cancers of pancreas, lung, breast and CLL yielding the area under the curve (AUC) values of 1.0000, 0.9952, 0.9800 and 0.9400, respectively. Narrowing to a single CpG site within the gene's promoter or four consecutive CpG units of the highest methylation levels within the first exon improved the detection power. GHSR hypermethylation was detected already at the early stage tumors. The accurate performance of this marker was further replicated in an independent set of pancreatic cancer and control samples (n = 78). These findings support the candidature of GHSR methylation as a highly accurate pan-cancer marker.

Involved external institutions

Deutsches Krebsforschungszentrum (DKFZ) DE Germany (DE) Zhukovsky – Gagarin Air Force Academy RU Russian Federation (RU) National Research Center for Hematology RU Russian Federation (RU) Odessa National Medical University UA Ukraine (UA) Universitätsklinikum Heidelberg DE Germany (DE) Ruprecht-Karls-Universität Heidelberg DE Germany (DE)

How to cite

APA:

Moskalev, E.A., Jandaghi, P., Fallah, M., Manoochehri, M., Botla, S.K., Kolychev, O.V.,... Riazalhosseini, Y. (2015). GHSR DNA hypermethylation is a common epigenetic alteration of high diagnostic value in a broad spectrum of cancers. Oncotarget, 6(6), 4418-27. https://doi.org/10.18632/oncotarget.2759

MLA:

Moskalev, Evgeny A., et al. "GHSR DNA hypermethylation is a common epigenetic alteration of high diagnostic value in a broad spectrum of cancers." Oncotarget 6.6 (2015): 4418-27.

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