Cutting-edge microangio-CT: new dimensions in vascular imaging and kidney morphometry
Hlushchuk R, Zubler C, Barré S, Shokiche CC, Schaad L, Röthlisberger R, Wnuk M, Daniel C, Khoma O, Tschanz SA, Reyes M, Djonov V (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Pages Range: ajprenal000992017
DOI: 10.1152/ajprenal.00099.2017
Abstract
In the last decades the contrast-enhanced microCT-imaging of whole animal kidney became increasingly important. The visualization was mainly limited to middle-sized vessels. Since modern desktop microCT-scanners provide the necessary detail resolution, we developed an approach for rapid visualization and consistent assessment of kidney vasculature and glomeruli number. This method is based on ?Angiofil®, a new polymerizing contrast agent with homogenous X-ray absorption, which provides continuous filling of the complete vasculature and enables correlative imaging approaches. For rapid and reliable kidney morphometry the microangioCT-datasets from GDNF+/- mice and their wildtype littermates were used. The results were obtained much faster compared to the current gold standard, histology-based stereology, and without processing artefacts. The histology-based morphometry was afterwards done on the same kidneys. Both approaches revealed that the GDNF+/- male mice had more than 30% fewer glomeruli. Futhermore, our approach allows defining sites of interest for further histological investigation, i.e., correlative morphology. The polymerized ?Angiofil stays in perfused vessels and is auto-fluorescent what greatly facilitates matching of histological sections with microangioCT-data. The presented approach is time-efficient and reliable qualitative and quantitative methodology. Besides glomerular morphometry the microangioCT-data can be used for qualitative and quantitative analysis of the kidney vasculature and correlative morphology.
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Switzerland (CH)How to cite
APA:
Hlushchuk, R., Zubler, C., Barré, S., Shokiche, C.C., Schaad, L., Röthlisberger, R.,... Djonov, V. (2017). Cutting-edge microangio-CT: new dimensions in vascular imaging and kidney morphometry. American Journal of Physiology-Renal Physiology, ajprenal000992017. https://doi.org/10.1152/ajprenal.00099.2017
MLA:
Hlushchuk, Ruslan, et al. "Cutting-edge microangio-CT: new dimensions in vascular imaging and kidney morphometry." American Journal of Physiology-Renal Physiology (2017): ajprenal000992017.
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