Vitamin E reduces TGF-beta2-induced changes in human trabecular meshwork cells
Yu AL, Moriniere J, Welge-Lussen U (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 38
Pages Range: 952-8
Journal Issue: 9
DOI: 10.3109/02713683.2013.793360
Abstract
To determine the effects of vitamin E on transforming growth factor-beta2 (TGF-?2)-induced cellular changes in cultured human trabecular meshwork (TM) cells.Human TM cells were pre-treated with different concentrations of vitamin E. Afterwards, cells were exposed to 1.0 ng/ml TGF-?2 for 24 h. Expressions of the heat shock protein ?B-crystallin, the extracellular matrix (ECM) component fibronectin and the ECM-degrading enzyme matrix metalloproteinase-2 (MMP-2) were examined by real-time polymerase chain reaction (PCR) analyses. The cytoskeleton was investigated by phalloidin staining.TGF-?2 increased the expressions of ?B-crystallin and fibronectin and reduced the levels of MMP-2. TGF-?2 induced the formation of actin stress fibers and cross-linked actin networks. Pre-treatment with different concentrations of vitamin E reversed the TGF-?2-induced cellular changes in cultured human TM cells.TGF-?2-mediated changes in human TM cells could be reduced by pre-treatment with vitamin E. Therefore, it may be speculated that increasing the antioxidative capacity may help to lower the incidence of characteristic glaucomatous changes in the TM.
Involved external institutions
Germany (DE)How to cite
APA:
Yu, A.L., Moriniere, J., & Welge-Lussen, U. (2013). Vitamin E reduces TGF-beta2-induced changes in human trabecular meshwork cells. Current Eye Research, 38(9), 952-8. https://dx.doi.org/10.3109/02713683.2013.793360
MLA:
Yu, Alice L., Jerome Moriniere, and Ulrich Welge-Lussen. "Vitamin E reduces TGF-beta2-induced changes in human trabecular meshwork cells." Current Eye Research 38.9 (2013): 952-8.
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