Pathogenesis of involutional ectropion and entropion: the involvement of matrix metalloproteinases in elastic fiber degradation

Damasceno RW, Heindl LM, Hofmann-Rummelt C, Belfort R, Schlötzer-Schrehardt U, Kruse F, Holbach LM (2011)

Publication Type: Journal article

Publication year: 2011

Journal

Orbit Taylor & Francis

Book Volume: 30

Pages Range: 132-9

Journal Issue: 3

DOI: 10.3109/01676830.2011.569049

Abstract

Purpose: To determine the elastic fiber content and ultrastructure as well as the expression of elastin-degrading enzymes in biopsy specimens from patients with involutional ectropion and entropion. Materials and Methods: Twenty consecutive patients with involutional ectropion (group 1) and twenty consecutive patients with entropion (group 2) were matched with twenty control patients (basal cell carcinoma) regarding age and gender. Full-thickness eyelid resections performed in study and control patients were examined by light and transmission electron microscopy, computer-assisted measurements, and immunohistochemistry using antibodies against matrix metalloproteinase (MMP)-2, MMP- 7, and MMP-9. The Kruskal-Wallis test and the Pearson chi-square test were performed. Results: Histopathologic analysis of the surgical specimens from patients with involutional ectropion and entropion showed a significant loss of elastic fibers in the eyelid skin, the pretarsal orbicularis oculi muscle, the perimeibomian tarsal stroma, and the intermeibomian tarsal stroma (P < 0.001). Residual elastic fibers revealed an abnormal ultrastructure. Immunohistochemistry demonstrated a significant overexpression of MMP- 2, MMP-7, and MMP-9 in the eyelid skin, the pretarsal orbicularis oculi muscle, the perimeibomian tarsal stroma, the intermeibomian tarsal stroma, and the conjunctiva in groups 1 and 2 compared to controls (P < 0.001). Conclusions: The present findings indicate that upregulation of elastolytic enzymes contributes to elastic fibre degradation in patients with involutional ectropion and entropion.

Authors with CRIS profile

Ursula Schlötzer-Schrehardt Medizinische Fakultät Friedrich Kruse Lehrstuhl für Augenheilkunde

Involved external institutions

Federal University of São Paulo / Universidade Federal de São Paulo (UNIFESP) BR Brazil (BR)

How to cite

APA:

Damasceno, R.W., Heindl, L.M., Hofmann-Rummelt, C., Belfort, R., Schlötzer-Schrehardt, U., Kruse, F., & Holbach, L.M. (2011). Pathogenesis of involutional ectropion and entropion: the involvement of matrix metalloproteinases in elastic fiber degradation. Orbit, 30(3), 132-9. https://doi.org/10.3109/01676830.2011.569049

MLA:

Damasceno, Renato W., et al. "Pathogenesis of involutional ectropion and entropion: the involvement of matrix metalloproteinases in elastic fiber degradation." Orbit 30.3 (2011): 132-9.

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