Identification of murine basophils by flow cytometry and histology

Schwartz C, Vöhringer D (2014)


Publication Type: Journal article

Publication year: 2014

Journal

Publisher: Humana Press, Inc.

Book Volume: 1192

Pages Range: 229-37

DOI: 10.1007/978-1-4939-1173-8_17

Abstract

Here we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4(-)CD19(-)CD49b(+)IgE(+) cells in flow cytometric analysis. When IgE levels are low one can also use anti-Fc?RI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.

Authors with CRIS profile

How to cite

APA:

Schwartz, C., & Vöhringer, D. (2014). Identification of murine basophils by flow cytometry and histology. Methods in Molecular Biology, 1192, 229-37. https://doi.org/10.1007/978-1-4939-1173-8_17

MLA:

Schwartz, Christian, and David Vöhringer. "Identification of murine basophils by flow cytometry and histology." Methods in Molecular Biology 1192 (2014): 229-37.

BibTeX: Download