Two-stage cultivation of Digitalis lanata cells: Semicontinuous production of deacetyllanatoside C in 20-litre airlift bioreactors.
Kreis W, Reinhard E (1990)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 1990
Journal
Publisher: Elsevier
Book Volume: 16
Pages Range: 123-136
DOI: 10.1016/0168-1656(90)90070-R
Abstract
A two-stage cultivation method was employed to develop a semicontinuous biotransformation process for the production of deacetyllanatoside C, a cardenolide of the important digoxin series. Digitoxin was used as the substrate for biotransformation. The process was optimized in 1-l shake flasks and then established on the 20-l scale using two airlift bioreactors, one for cell growth (working volume 12 litres) and another for deacetyllanatoside C production (working volume 18 litres). Growth and production phases were synchronized and the process finally ran semicontinuously in 7-d cycles. Six consecutive production runs were performed yielding a total of 43.8 g deacetyllanatoside C. © 1990.
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APA:
Kreis, W., & Reinhard, E. (1990). Two-stage cultivation of Digitalis lanata cells: Semicontinuous production of deacetyllanatoside C in 20-litre airlift bioreactors. Journal of Biotechnology, 16, 123-136. https://doi.org/10.1016/0168-1656(90)90070-R
MLA:
Kreis, Wolfgang, and Ernst Reinhard. "Two-stage cultivation of Digitalis lanata cells: Semicontinuous production of deacetyllanatoside C in 20-litre airlift bioreactors." Journal of Biotechnology 16 (1990): 123-136.
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