Synergic Functions of miRNAs Determine Neuronal Fate of Adult Neural Stem Cells

Pons-Espinal M, De Luca E, Marzi MJ, Beckervordersandforth R, Armirotti A, Nicassio F, Fabel K, Kempermann G, Tonelli DDP (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Stem Cell Reports Elsevier

Book Volume: 8

Pages Range: 1046-1061

Journal Issue: 4

DOI: 10.1016/j.stemcr.2017.02.012

Abstract

Adult neurogenesis requires the precise control of neuronal versus astrocyte lineage determination in neural stem cells. While microRNAs (miRNAs) are critically involved in this step during development, their actions in adult hippocampal neural stem cells (aNSCs) has been unclear. As entry point to address that question we chose DICER, an endoribonuclease essential for miRNA biogenesis and other RNAi-related processes. By specific ablation of Dicer in aNSCs in vivo and in vitro, we demonstrate that miRNAs are required for the generation of new neurons, but not astrocytes, in the adult murine hippocampus. Moreover, we identify 11 miRNAs, of which 9 have not been previously characterized in neurogenesis, that determine neurogenic lineage fate choice of aNSCs at the expense of astrogliogenesis. Finally, we propose that the 11 miRNAs sustain adult hippocampal neurogenesis through synergistic modulation of 26 putative targets from different pathways.

Authors with CRIS profile

Ruth Beckervordersandforth-Bonk Lehrstuhl für Biochemie und Pathobiochemie

Involved external institutions

Technische Universität Dresden DE Germany (DE) Italian Institute of Technology / Istituto Italiano di Tecnologia (IIT) IT Italy (IT) Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) DE Germany (DE)

How to cite

APA:

Pons-Espinal, M., De Luca, E., Marzi, M.J., Beckervordersandforth, R., Armirotti, A., Nicassio, F.,... Tonelli, D.D.P. (2017). Synergic Functions of miRNAs Determine Neuronal Fate of Adult Neural Stem Cells. Stem Cell Reports, 8(4), 1046-1061. https://doi.org/10.1016/j.stemcr.2017.02.012

MLA:

Pons-Espinal, Meritxell, et al. "Synergic Functions of miRNAs Determine Neuronal Fate of Adult Neural Stem Cells." Stem Cell Reports 8.4 (2017): 1046-1061.

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