Naito-Matsui Y, Takada S, Kano Y, Iyoda T, Sugai M, Shimizu A, Inaba K, Nitschke L, Tsubata T, Oka S, Kozutsumi Y, Takematsu H (2014)
Publication Status: Published
Publication Type: Journal article
Publication year: 2014
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Book Volume: 289
Pages Range: 1564-1579
Journal Issue: 3
Sialic acids (Sias) are often conjugated to the termini of cellular glycans and are key mediators of cellular recognition. Sias are nine-carbon acidic sugars, and, in vertebrates, the major species are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), differing in structure at the C5 position. Previously, we described a positive feedback loop involving regulation of Neu5Gc expression in mouse B cells. In this context, Neu5Gc negatively regulated B-cell proliferation, and Neu5Gc expression was suppressed upon activation. Similarly, resting mouse T cells expressed principally Neu5Gc, and Neu5Ac was induced upon activation. In the present work, we used various probes to examine sialoglycan expression by activated T cells in terms of the Sia species expressed and the linkages of Sias to glycans. Upon T-cell activation, sialoglycan expression shifted from Neu5Gc to Neu5Ac, and the linkage shifted from 2,6 to 2,3. These changes altered the expression levels of sialic acid-binding immunoglobulin-like lectin (siglec) ligands. Expression of sialoadhesin and Siglec-F ligands increased, and that of CD22 ligands decreased. Neu5Gc exerted a negative effect on T-cell activation, both in terms of the proliferative response and in the context of activation marker expression. Suppression of Neu5Gc expression in mouse T and B cells prevented the development of nonspecific CD22-mediated T cell-B cell interactions. Our results suggest that an activation-dependent shift from Neu5Gc to Neu5Ac and replacement of 2,6 by 2,3 linkages may regulate immune cell interactions at several levels.
APA:
Naito-Matsui, Y., Takada, S., Kano, Y., Iyoda, T., Sugai, M., Shimizu, A.,... Takematsu, H. (2014). Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells. Journal of Biological Chemistry, 289(3), 1564-1579. https://doi.org/10.1074/jbc.M113.523753
MLA:
Naito-Matsui, Yuko, et al. "Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells." Journal of Biological Chemistry 289.3 (2014): 1564-1579.
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