Krappmann S (2017)
Publication Type: Journal article, Review article
Publication year: 2017
Book Volume: 55
Pages Range: 16-23
Journal Issue: 1
DOI: 10.1093/mmy/myw097
Research on fungal pathogens with the aim to identify virulence determinants strictly relies on the generation of defined, recombinant strains, a task that is executed by means of a sophisticated molecular biology toolbox. Recent developments in fungal genome engineering have opened a new frontier by implementing the CRISPR-Cas9 technology, based on expression of the Cas9 endonuclease that is loaded by a single guiding RNA (sgRNA) molecule to target a defined site in the recipient genome. This novel approach has been adapted successfully to engineer fungal genomes, among them the one of the human-pathogenic mould Aspergillus fumigatus Implementation of the required components was achieved by various means that differ with respect to expression of the Cas9 enzyme and sgRNA delivery. Validation of CRISPR-Cas9-mediated mutagenesis could be executed by targeting selected candidate genes of A. fumigatus to provide a promising perspective for screening and multiplexing approaches to scrutinize the virulome of this opportunistic fungal pathogen in a comprehensive manner, such as by analyzing genetic polymorphisms or the function of gene families.
APA:
Krappmann, S. (2017). CRISPR-Cas9, the new kid on the block of fungal molecular biology. Medical Mycology, 55(1), 16-23. https://doi.org/10.1093/mmy/myw097
MLA:
Krappmann, Sven. "CRISPR-Cas9, the new kid on the block of fungal molecular biology." Medical Mycology 55.1 (2017): 16-23.
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