Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble
Del Carmen Montero-Calasanz M, Hofner B, Goeker M, Rohde M, Sproeer C, Hezbri K, Gtari M, Schumann P, Klenk HP (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Hindawi Publishing Corporation
Book Volume: 2014
Pages Range: 914767
DOI: 10.1155/2014/914767
Abstract
A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18T, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15-35°C, at pH 5.5-9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1 ?8c and C16:1 ?7c. The 16S rRNA gene showed 97.4-99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18T is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18T (=DSM 44209T=CCUG 63018T). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.
Authors with CRIS profile
Involved external institutions
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures / Deutsche Sammlung von Mikroorganismen und Zellkulturen
Germany (DE)
Helmholtz-Zentrum für Infektionsforschung (HZI)
Germany (DE)
Tunis El Manar University (UTM) / L'université de Tunis - El Manar جامعة تونس المنار
Tunisia (TN)
Germany (DE)
Helmholtz-Zentrum für Infektionsforschung (HZI)
Germany (DE)
Tunis El Manar University (UTM) / L'université de Tunis - El Manar جامعة تونس المنار
Tunisia (TN)
How to cite
APA:
Del Carmen Montero-Calasanz, M., Hofner, B., Goeker, M., Rohde, M., Sproeer, C., Hezbri, K.,... Klenk, H.-P. (2014). Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble. BioMed Research International, 2014, 914767. https://doi.org/10.1155/2014/914767
MLA:
Del Carmen Montero-Calasanz, Maria, et al. "Geodermatophilus poikilotrophi sp. nov.: a multitolerant actinomycete isolated from dolomitic marble." BioMed Research International 2014 (2014): 914767.
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