Numerical analysis of Ca2+ depletion in the transverse tubular system of mammalian muscle.

Friedrich O, Ehmer T, Uttenweiler D, Vogel M, Barry PH, Fink R (2001)

Publication Status: Published

Publication Type: Journal article

Publication year: 2001

Journal

Biophysical Journal Elsevier (Cell Press) / Biophysical Society

Publisher: Elsevier (Cell Press) / Biophysical Society

Book Volume: 80

Pages Range: 2046-55

Journal Issue: 5

DOI: 10.1016/S0006-3495(01)76178-4

Abstract

Calcium currents were recorded in contracting and actively shortening mammalian muscle fibers. In order to characterize the influence of extracellular calcium concentration changes in the small unstirred lumina of the transverse tubular system (TTS) on the time course of the slow L-type calcium current (I(Ca)), we have combined experimental measurements of I(Ca) with quantitative numerical simulations of Ca2+ depletion. I(Ca) was recorded both in calcium-buffered and unbuffered external solutions using the two-microelectrode voltage clamp technique (2-MVC) on short murine toe muscle fibers. A simulation program based on a distributed TTS model was used to calculate the effect of ion depletion in the TTS. The experimental data obtained in a solution where ion depletion is suppressed by a high amount of a calcium buffering agent were used as input data for the simulation. The simulation output was then compared with experimental data from the same fiber obtained in unbuffered solution. Taking this approach, we could quantitatively show that the calculated Ca2+ depletion in the transverse tubular system of contracting mammalian muscle fibers significantly affects the time-dependent decline of Ca2+ currents. From our findings, we conclude that ion depletion in the tubular system may be one of the major effects for the I(Ca) decline measured in isotonic physiological solution under voltage clamp conditions.

Authors with CRIS profile

Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

University of New South Wales (UNSW) AU Australia (AU) Ruprecht-Karls-Universität Heidelberg DE Germany (DE)

How to cite

APA:

Friedrich, O., Ehmer, T., Uttenweiler, D., Vogel, M., Barry, P.H., & Fink, R. (2001). Numerical analysis of Ca2+ depletion in the transverse tubular system of mammalian muscle. Biophysical Journal, 80(5), 2046-55. https://doi.org/10.1016/S0006-3495(01)76178-4

MLA:

Friedrich, Oliver, et al. "Numerical analysis of Ca2+ depletion in the transverse tubular system of mammalian muscle." Biophysical Journal 80.5 (2001): 2046-55.

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