Influence of precursors and inhibitors of the sterol pathway on sterol and cardenolide metabolism in Digitalis lanata EHRH
Milek F, Reinhard E, Kreis W (1997)
Publication Type: Journal article, Original article
Publication year: 1997
Journal
Publisher: Elsevier
Book Volume: 35
Pages Range: 111-121
Journal Issue: 2
Abstract
The influence of 25-azacycloartanol, an inhibitor of SAM:cycloartenol 24-methyltransferase, and cyproconazole, an experimental triazole known to inhibit ergosterol biosynthesis in fungi, on cardenolide and phytosterol accumulation was studied in different Digitalis lanata Ehrh, tissue cultures. The influence of various steroids was also examined. Cardenolide-producing as well as cardenolide-free tissue cultures contained free cholesterol, sitosterol, campesterol and stigmasterol. Stigmasterol was the predominant sterol in cardenolide-producing cultures, whereas sitosterol dominated in cardenolide-free tissues. Suspension-cultured cells had the highest sterol content of the materials analyzed. Cardenolides and 24-alkyl sterols decreased after administration of 25-azacycloartanol, whereas the pool of free cholesterol increased. Cyproconazol influenced mainly the pools of 24-alkyl sterols and cardenolides; cholesterol contents did not change significantly. The specificity and toxicity of the inhibitors applied were evaluated. Neither the 12β-hydroxylation of β-methyldigitoxin was influenced, nor was the stimulating effect of 21-hydroxypregnenolone on cardenolide formation inhibited. The results presented support the view that 24-alkyl sterols rather than cholesterol are the favoured sterol precursors of the cardenolides in Digitalis lanata.
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Germany (DE)How to cite
APA:
Milek, F., Reinhard, E., & Kreis, W. (1997). Influence of precursors and inhibitors of the sterol pathway on sterol and cardenolide metabolism in Digitalis lanata EHRH. Plant Physiology and Biochemistry, 35(2), 111-121.
MLA:
Milek, Frank, Ernst Reinhard, and Wolfgang Kreis. "Influence of precursors and inhibitors of the sterol pathway on sterol and cardenolide metabolism in Digitalis lanata EHRH." Plant Physiology and Biochemistry 35.2 (1997): 111-121.
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