3D high-speed multifocal multiphoton microscopy in muscle and wavelet analysis of fast elementary calcium release events

Von Wegner F, Both M, Fink R, Friedrich O (2006)

Publication Language: Arabic

Publication Status: Published

Publication Type: Conference contribution, Conference Contribution

Publication year: 2006

Book Volume: 2006

Pages Range: 1148-1151

Article Number: 1625126

Event location: Arlington, VA

ISBN: 9780780395770

URI: https://www.scopus.com/record/display.uri?eid=2-s2.0-33750959403&origin=inward

Abstract

Recent approaches to record and analyze skeletal muscle elementary calcium release events (ECRE) in three dimensions (XYT) were restricted to short time series and small areas of interest. Here, we present a combined method for XYT analysis of ECRE using high-speed multifocal multiphoton calcium imaging and a wavelet based automated denoising and detection algorithm. Images were analyzed with the 'à trous' implementation of the wavelet transform. 'Hardthreshold' wavelet denoising resulted in markedly improved signal-to-noise ratios compared to low-pass filtering. Automated detection of ECRE was performed across wavelet scales and single events were tracked within an image sequence using 3D segmentation. With this combined approach, the morphological dynamics of fast ECRE in mammalian skeletal muscle fibres could be faithfully monitored with high spatial and temporal resolution. Our method may provide an improved tool for the facilitated detection of ECRE in muscle in three dimensions even under conditions of undisturbed calcium homeostasis. © 2006 IEEE.

Authors with CRIS profile

Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE)

How to cite

APA:

Von Wegner, F., Both, M., Fink, R., & Friedrich, O. (2006). 3D high-speed multifocal multiphoton microscopy in muscle and wavelet analysis of fast elementary calcium release events. In Proceedings of the 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro (pp. 1148-1151). Arlington, VA.

MLA:

Von Wegner, Frederic, et al. "3D high-speed multifocal multiphoton microscopy in muscle and wavelet analysis of fast elementary calcium release events." Proceedings of the 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Arlington, VA 2006. 1148-1151.

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