Rohrbach P, Sanchez CP, Hayton K, Friedrich O, Patel J, Singh Sidhu AB, Ferdig MT, Fidock DA, Lanzer M (2006)
Publication Status: Published
Publication Type: Journal article
Publication year: 2006
Publisher: Nature Publishing Group: EMBO
Book Volume: 25
Pages Range: 3000-11
Journal Issue: 13
The P-glycoprotein homolog of the human malaria parasite Plasmodium falciparum (Pgh-1) has been implicated in decreased susceptibility to several antimalarial drugs, including quinine, mefloquine and artemisinin. Pgh-1 mainly resides within the parasite's food vacuolar membrane. Here, we describe a surrogate assay for Pgh-1 function based on the subcellular distribution of Fluo-4 acetoxymethylester and its free fluorochrome. We identified two distinct Fluo-4 staining phenotypes: preferential staining of the food vacuole versus a more diffuse staining of the entire parasite. Genetic, positional cloning and pharmacological data causatively link the food vacuolar Fluo-4 phenotype to those Pgh-1 variants that are associated with altered drug responses. On the basis of our data, we propose that Pgh-1 imports solutes, including certain antimalarial drugs, into the parasite's food vacuole. The implications of our findings for drug resistance mechanisms and testing are discussed.
APA:
Rohrbach, P., Sanchez, C.P., Hayton, K., Friedrich, O., Patel, J., Singh Sidhu, A.B.,... Lanzer, M. (2006). Genetic linkage of pfmdr1 with food vacuolar solute import in Plasmodium falciparum. EMBO Journal, 25(13), 3000-11. https://doi.org/10.1038/sj.emboj.7601203
MLA:
Rohrbach, Petra, et al. "Genetic linkage of pfmdr1 with food vacuolar solute import in Plasmodium falciparum." EMBO Journal 25.13 (2006): 3000-11.
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