Multiresolution transform denoising and segmentation of single molecule motility image series

Friedrich O, von Wegner F, Ober T, Weber C, Fink R, Vogel M (2007)

Publication Status: Published

Publication Type: Conference contribution, Conference Contribution

Publication year: 2007

Book Volume: 6626

Article Number: 66260Q

Event location: Munich

ISBN: 9780819467706

DOI: 10.1117/12.728371

Abstract

We present a multiresolution transform-based method for the extraction of moving filament trajectories from single molecule motility data. Noise-corrupted fluorescence image series are denoised using the multiscale median transform and trajectories are detected in the denoised data set. The presented method reduces noise more efficiently than 2D-anisotropic diffusion and several wavelet based techniques. Fibre trajectories are extracted by segmentation of the denoised image stacks and non-crossing trajectories are unambiguously identified combining the information of 2D (XY) and 3D (XYT) segmentation. The algorithm is applied and evaluated using experimental data sets - image sequences of fluorescently labeled F-actin molecules and their 2D-trajectories on a myosin coated surface. This so-called 'motility assay' is used to analyse kinetics, biochemical regulation and pharmacological modulation of these biologically relevant molecules. The presented method improves signal-to-background discrimination, facilitates filament identification and finally, may contribute to significantly improve the performance of this assay.

Authors with CRIS profile

Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE)

How to cite

APA:

Friedrich, O., von Wegner, F., Ober, T., Weber, C., Fink, R., & Vogel, M. (2007). Multiresolution transform denoising and segmentation of single molecule motility image series. In Proceedings of the Molecular Imaging. Munich.

MLA:

Friedrich, Oliver, et al. "Multiresolution transform denoising and segmentation of single molecule motility image series." Proceedings of the Molecular Imaging, Munich 2007.

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