IL-9 regulates intestinal barrier function in experimental T cell-mediated colitis
Gerlach K, McKenzie AN, Neurath M, Weigmann B (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 3
Pages Range: e983777
Journal Issue: 1-2
DOI: 10.4161/21688370.2014.983777
Abstract
As previous studies suggested that IL-9 may control intestinal barrier function, we tested the role of IL-9 in experimental T cell-mediated colitis induced by the hapten reagent 2,4,6-trinitrobenzenesulfonic acid (TNBS). The deficiency of IL-9 suppressed TNBS-induced colitis and led to lower numbers of PU.1 expressing T cells in the lamia propria, suggesting a regulatory role for Th9 cells in the experimental TNBS colitis model. Since IL-9 is known to functionally alter intestinal barrier function in colonic inflammation, we assessed the expression of tight junction molecules in intestinal epithelial cells of TNBS-inflamed mice. Therefore we made real-time PCR analyses for tight junction molecules in the inflamed colon from wild-type and IL-9 KO mice, immunofluorescent stainings and investigated the expression of junctional proteins directly in intestinal epithelial cells of TNBS-inflamed mice by Western blot studies. The results demonstrated that sealing proteins like occludin were up regulated in the colon of inflamed IL-9 KO mice. In contrast, the tight junction protein Claudin1 showed lower expression levels when IL-9 is absent. Surprisingly, the pore-forming molecule Claudin2 revealed equal expression in TNBS-treated wild-type and IL-9-deficient animals. These results illustrate the pleiotropic functions of IL-9 in changing intestinal permeability in experimental colitis. Thus, modulation of IL-9 function emerges as a new approach for regulating barrier function in intestinal inflammation.
Authors with CRIS profile
Katharina Gerlach
Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology
Markus Neurath
Lehrstuhl für Innere Medizin I
Benno Weigmann
Medizinische Fakultät
Involved external institutions
Germany (DE)How to cite
APA:
Gerlach, K., McKenzie, A.N., Neurath, M., & Weigmann, B. (2015). IL-9 regulates intestinal barrier function in experimental T cell-mediated colitis. Tissue Barriers, 3(1-2), e983777. https://doi.org/10.4161/21688370.2014.983777
MLA:
Gerlach, Katharina, et al. "IL-9 regulates intestinal barrier function in experimental T cell-mediated colitis." Tissue Barriers 3.1-2 (2015): e983777.
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