Multi-colour FISH in oesophageal adenocarcinoma-predictors of prognosis independent of stage and grade
Geppert CI, Ruemmele P, Sarbia M, Langer R, Feith M, Morrison L, Pestova E, Schneider-Stock R, Hartmann A, Rau T (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Cancer Research UK
Book Volume: 110
Pages Range: 2985-95
Journal Issue: 12
DOI: 10.1038/bjc.2014.238
Abstract
Oesophageal adenocarcinoma or Barrett's adenocarcinoma (EAC) is increasing in incidence and stratification of prognosis might improve disease management. Multi-colour fluorescence in situ hybridisation (FISH) investigating ERBB2, MYC, CDKN2A and ZNF217 has recently shown promising results for the diagnosis of dysplasia and cancer using cytological samples.To identify markers of prognosis we targeted four selected gene loci using multi-colour FISH applied to a tissue microarray containing 130 EAC samples. Prognostic predictors (P1, P2, P3) based on genomic copy numbers of the four loci were statistically assessed to stratify patients according to overall survival in combination with clinical data.The best stratification into favourable and unfavourable prognoses was shown by P1, percentage of cells with less than two ZNF217 signals; P2, percentage of cells with fewer ERBB2- than ZNF217 signals; and P3, overall ratio of ERBB2-/ZNF217 signals. Median survival times for P1 were 32 vs 73 months, 28 vs 73 months for P2; and 27 vs 65 months for P3. Regarding each tumour grade P2 subdivided patients into distinct prognostic groups independently within each grade, with different median survival times of at least 35 months.Cell signal number of the ERBB2 and ZNF217 loci showed independence from tumour stage and differentiation grade. The prognostic value of multi-colour FISH-assays is applicable to EAC and is superior to single markers.
Authors with CRIS profile
Carol-Immanuel Geppert
Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie
Regine Schneider-Stock
Professur für Experimentelle Tumorpathologie
Arndt Hartmann
Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie
Involved external institutions
Universitätsklinikum Regensburg
Germany (DE)
Universität Bern
Switzerland (CH)
Technische Universität München (TUM)
Germany (DE)
Ventana Medical Systems, Inc
United States (USA) (US)
Abbott Molecular
United States (USA) (US)
Germany (DE)
Universität Bern
Switzerland (CH)
Technische Universität München (TUM)
Germany (DE)
Ventana Medical Systems, Inc
United States (USA) (US)
Abbott Molecular
United States (USA) (US)
How to cite
APA:
Geppert, C.-I., Ruemmele, P., Sarbia, M., Langer, R., Feith, M., Morrison, L.,... Rau, T. (2014). Multi-colour FISH in oesophageal adenocarcinoma-predictors of prognosis independent of stage and grade. British Journal of Cancer, 110(12), 2985-95. https://doi.org/10.1038/bjc.2014.238
MLA:
Geppert, Carol-Immanuel, et al. "Multi-colour FISH in oesophageal adenocarcinoma-predictors of prognosis independent of stage and grade." British Journal of Cancer 110.12 (2014): 2985-95.
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