Acetyl Coenzyme A:Digitoxin 15' -O-Acetyltransferase from Digitalis Plants and Suspension Cultures

Sutor R, Kreis W, Hoelz H, Reinhard E (1993)


Publication Language: English

Publication Type: Journal article, Original article

Publication year: 1993

Journal

Publisher: Elsevier

Book Volume: 32

Pages Range: 569-573

Journal Issue: 3

DOI: 10.1016/S0031-9422(00)95138-0

Abstract

The enzymatic acetylation of cardenolides was investigated. Suspension cultures of Digitalis grandiflora converted digitoxin to acetylated cardenolides such as α-acetyldigitoxin and lanatoside A. Protein preparations from the same source catalysed the transfer of an acetyl group from acetyl coenzyme A to the 15′-O-position of digitoxin or digoxin. The enzyme involved, acetyl coenzyme A: digitoxin 15′-O-acetyltransferase, was characterized and detected in protein extracts from other tissues, namely the leaves of D. grandiflora, as well as the leaves, callus and suspended cells of D. lanata. Compartmentalization studies with isolated protoplasts and vacuoles revealed that the digitoxin acetyltransferase is a soluble cytosolic enzyme. © 1993.

Authors with CRIS profile

Involved external institutions

How to cite

APA:

Sutor, R., Kreis, W., Hoelz, H., & Reinhard, E. (1993). Acetyl Coenzyme A:Digitoxin 15' -O-Acetyltransferase from Digitalis Plants and Suspension Cultures. Phytochemistry, 32(3), 569-573. https://doi.org/10.1016/S0031-9422(00)95138-0

MLA:

Sutor, Renate, et al. "Acetyl Coenzyme A:Digitoxin 15' -O-Acetyltransferase from Digitalis Plants and Suspension Cultures." Phytochemistry 32.3 (1993): 569-573.

BibTeX: Download