Destabilization of the human epigenome: consequences of foreign DNA insertions
Weber S, Hofmann A, Herms S, Hoffmann P, Dörfler W (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 7
Pages Range: 745-55
Journal Issue: 5
DOI: 10.2217/epi.15.40
Abstract
We previously reported changes of DNA methylation and transcription patterns in mammalian cells that carry integrated foreign DNA. Experiments were now designed to assess the epigenetic consequences of inserting a 5.6 kbp plasmid into the human genome.Differential transcription and CpG methylation patterns were compared between transgenomic and nontransgenomic cell clones by using gene chip microarray systems.In 4.7% of the 28.869 gene segments analyzed, transcriptional activities were up- or downregulated in the transgenomic cell clones. Genome-wide profiling revealed differential methylation in 3791 of > 480,000 CpG's examined in transgenomic versus nontransgenomic clones.The data document genome-wide effects of foreign DNA insertions on the epigenetic stability of human cells. Many fields in experimental biology and medicine employ transgenomic or otherwise genome-manipulated cells or organisms without considering the epigenetic consequences for the recipient genomes.
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Germany (DE)How to cite
APA:
Weber, S., Hofmann, A., Herms, S., Hoffmann, P., & Dörfler, W. (2015). Destabilization of the human epigenome: consequences of foreign DNA insertions. Epigenomics, 7(5), 745-55. https://doi.org/10.2217/epi.15.40
MLA:
Weber, Stefanie, et al. "Destabilization of the human epigenome: consequences of foreign DNA insertions." Epigenomics 7.5 (2015): 745-55.
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