Class I myosin Myo1e regulates TLR4-triggered macrophage spreading, chemokine release, and antigen presentation via MHC class II

Wenzel J, Ouderkirk JL, Krendel M, Lang R (2015)

Publication Type: Journal article

Publication year: 2015

Journal

European Journal of Immunology Wiley-VCH Verlag

Book Volume: 45

Pages Range: 225-37

Journal Issue: 1

DOI: 10.1002/eji.201444698

Abstract

TLR-mediated recognition of microbial danger induces substantial changes in macrophage migration, adherence, and phagocytosis. Recently, we described the LPS-regulated phosphorylation of many cytoskeleton-associated proteins by phosphoproteomics. The functional role of these cytoskeletal and motor proteins in innate immune cell responses is largely unexplored. Here, we first identified both long-tailed class I myosins Myo1e and Myo1f as important contributors to LPS-triggered macrophage spreading. Mouse bone marrow-derived macrophages and DCs deficient in Myo1e selectively secreted increased amounts of the chemokine CCL2. In addition, the cell surface expression of MHC class II (MHC-II) on both cell types was reduced in the absence of Myo1e. However, transcriptional changes in CCL2 and MHC-II were not observed in the absence of Myo1e, indicating that Myo1e regulates specific intracellular transport processes. The capacity of macrophages and DCs lacking Myo1e to stimulate antigen-specific CD4(+) T-cell proliferation was impaired, consistent with the reduced MHC-II surface protein levels. Surprisingly, in Myo1e-deficient DCs, the proteolytic cleavage of endocytosed antigen was also increased. Together, our results provide evidence for a non-redundant function of the motor protein Myo1e in the regulation of TLR4-controlled, cytoskeleton-associated functional properties of macrophages and DCs, and in induction of a full MHC-II-restricted adaptive immune response.

Authors with CRIS profile

Jens Wenzel Professur für Angeborene Immunität und Pathogenerkennung Roland Lang Professur für Angeborene Immunität und Pathogenerkennung

Involved external institutions

State University of New York at Albany (UNY Albany / UAlbany) US United States (USA) (US)

How to cite

APA:

Wenzel, J., Ouderkirk, J.L., Krendel, M., & Lang, R. (2015). Class I myosin Myo1e regulates TLR4-triggered macrophage spreading, chemokine release, and antigen presentation via MHC class II. European Journal of Immunology, 45(1), 225-37. https://doi.org/10.1002/eji.201444698

MLA:

Wenzel, Jens, et al. "Class I myosin Myo1e regulates TLR4-triggered macrophage spreading, chemokine release, and antigen presentation via MHC class II." European Journal of Immunology 45.1 (2015): 225-37.

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