Expression and Localization of Lung Surfactant Proteins in Human Testis

Beileke S, Claassen H, Wagner W, Matthies C, Ruf C, Hartmann A, Garreis F, Paulsen F, Schicht M, Bräuer L (2015)

Publication Type: Journal article

Publication year: 2015

Journal

PLoS ONE Public Library of Science

Book Volume: 10

Pages Range: e0143058

Journal Issue: 11

DOI: 10.1371/journal.pone.0143058

Abstract

Surfactant proteins (SPs) have been described in various tissues and fluids including tissues of the nasolacrimal apparatus, airways and digestive tract. Human testis have a glandular function as a part of the reproductive and the endocrine system, but no data are available on SPs in human testis and prostate under healthy and pathologic conditions.The aim of the study was the detection and characterization of the surfactant proteins A, B, C and D (SP-A, SP-B, SP-C, SP-D) in human testis. Additionally tissue samples affected by testicular cancer were investigated.Surfactant proteins A, B, C and D were detected using RT-PCR in healthy testis. By means of Western blot analysis, these SPs were detected at the protein level in normal testis, seminoma and seminal fluid, but not in spermatozoa. Expression of SPs was weaker in seminoma compared to normal testicular tissue. SPs were localized in combination with vimentin immunohistochemically in cells of Sertoli and Leydig.Surfactant proteins seem to be inherent part of the human testis. By means of physicochemical properties the proteins appear to play a role during immunological and rheological process of the testicular tissue. The presence of SP-B and SP-C in cells of Sertoli correlates with their function of fluid secretion and may support transportation of spermatozoa. In seminoma the expression of all SP's was generally weaker compared to normal germ cells. This could lead to a reduction of immunomodulatory and rheology processes in the germ cell tumor.

Authors with CRIS profile

Stephanie Beileke Lehrstuhl für Funktionelle und Klinische Anatomie Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Fabian Garreis Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie Lars Bräuer Professur für Oberflächenaktive Proteine

Involved external institutions

Bundeswehrkrankenhaus Hamburg DE Germany (DE) Martin-Luther-Universität Halle-Wittenberg (MLU) DE Germany (DE)

How to cite

APA:

Beileke, S., Claassen, H., Wagner, W., Matthies, C., Ruf, C., Hartmann, A.,... Bräuer, L. (2015). Expression and Localization of Lung Surfactant Proteins in Human Testis. PLoS ONE, 10(11), e0143058. https://doi.org/10.1371/journal.pone.0143058

MLA:

Beileke, Stephanie, et al. "Expression and Localization of Lung Surfactant Proteins in Human Testis." PLoS ONE 10.11 (2015): e0143058.

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