Fahlbusch FB, Menendez-Castro C, Nögel S, Marek I, Beckmann M, Schleussner E, Ruebner M, Huebner H, Dörr HG, Schild RL, Doetsch J, Rascher W, Hartner A (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 6
Pages Range: 317-26
Journal Issue: 4
DOI: 10.1017/S2040174415001154
Idiopathic intrauterine growth restriction (IUGR) is a result of impaired placental nutrient supply. Newborns with IUGR exhibiting postnatal catch-up growth are of higher risk for cardiovascular and metabolic co-morbidities in adult life. Mammalian target of rapamycin (mTOR) was recently shown to function as a placental nutrient sensor. Thus, we determined possible correlations of members of the placental mTOR signaling cascade with auxologic parameters of postnatal growth. The protein expression and activity of mTOR-pathway signaling components, Akt, AMP-activated protein kinase ?, mTOR, p70S6kinase1 and insulin receptor substrate-1 were analysed via western blotting in IUGR v. matched appropriate-for-gestational age (AGA) placentas. Moreover, mTOR was immunohistochemically stained in placental sections. Data from western blot analyses were correlated with retrospective auxological follow-up data at 1 year of age. We found significant catch-up growth in the 1st year of life in the IUGR group. MTOR and its activated form are immunohistochemically detected in multiple placental compartments. We identified correlations of placental mTOR-pathway signaling components to auxological data at birth and at 1 year of life in IUGR. Analysis of the protein expression and phosphorylation level of mTOR-pathway components in IUGR and AGA placentas postpartum, however, did not reveal pathognomonic changes. Our findings suggest that the level of activated mTOR correlates with early catch-up growth following IUGR. However, the complexity of signals converging at the mTOR nexus and its cellular distribution pattern seem to limit its potential as biomarker in this setting.
APA:
Fahlbusch, F.B., Menendez-Castro, C., Nögel, S., Marek, I., Beckmann, M., Schleussner, E.,... Hartner, A. (2015). The placental mTOR-pathway: correlation with early growth trajectories following intrauterine growth restriction? Journal of Developmental Origins of Health and Disease, 6(4), 317-26. https://doi.org/10.1017/S2040174415001154
MLA:
Fahlbusch, F. B., et al. "The placental mTOR-pathway: correlation with early growth trajectories following intrauterine growth restriction?" Journal of Developmental Origins of Health and Disease 6.4 (2015): 317-26.
BibTeX: Download