Murine CD83-positive T cells mediate suppressor functions in vitro and in vivo

Kreiser S, Eckhardt J, Kuhnt C, Stein MF, Krzyzak L, Seitz C, Tucher C, Knippertz I, Becker C, Günther C, Steinkasserer A, Lechmann M (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Immunobiology Elsevier

Book Volume: 220

Pages Range: 270-9

Journal Issue: 2

DOI: 10.1016/j.imbio.2014.08.005

Abstract

The CD83 molecule (CD83) is a well-known surface marker present on mature dendritic cells (mDC). In this study, we show that CD83 is also expressed on a subset of T cells which mediate regulatory T cell (Treg)-like suppressor functions in vitro and in vivo. Treg-associated molecules including CD25, cytotoxic T lymphocyte antigen-4 (CTLA-4), glucocorticoid-induced TNFR family-related gene (GITR), Helios and neuropilin-1 (NRP-1) as well as forkhead box protein 3 (FOXP3) were specifically expressed by these CD83(+) T cells. In contrast, CD83(-) T cells showed a naive T cell phenotype with effector T cell properties upon activation. Noteworthy, CD83(-) T cells were not able to upregulate CD83 despite activation. Furthermore, CD83(+) T cells suppressed the proliferation and inflammatory cytokine release of CD83(-) T cells in vitro. Strikingly, stimulated CD83(+) T cells released soluble CD83 (sCD83), which has been reported to possess immunosuppressive properties. In vivo, using the murine transfer colitis model we could show that CD83(+) T cells were able to suppress colitis symptoms while CD83(-) T cells possessed effector functions. In addition, this CD83 expression is also conserved on expanded human Treg. Thus, from these studies we conclude that CD83(+) T cells share important features with regulatory T cells, identifying CD83 as a novel lineage marker to discriminate between different T cell populations.

Authors with CRIS profile

Simon Kreiser Professur für Experimentelle Dermatologie Lena Krzyzak Professur für Experimentelle Dermatologie Christoph Becker Professur für Molekulare Gastroenterologie Claudia Günther Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Alexander Steinkasserer Professur für Experimentelle Dermatologie Matthias Lechmann Department of Immune Modulation

How to cite

APA:

Kreiser, S., Eckhardt, J., Kuhnt, C., Stein, M.F., Krzyzak, L., Seitz, C.,... Lechmann, M. (2015). Murine CD83-positive T cells mediate suppressor functions in vitro and in vivo. Immunobiology, 220(2), 270-9. https://doi.org/10.1016/j.imbio.2014.08.005

MLA:

Kreiser, Simon, et al. "Murine CD83-positive T cells mediate suppressor functions in vitro and in vivo." Immunobiology 220.2 (2015): 270-9.

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