miR-200c dampens cancer cell migration via regulation of protein kinase A subunits

Sigloch FC, Burk UC, Biniossek ML, Brabletz T, Schilling O (2015)


Publication Type: Journal article

Publication year: 2015

Journal

Book Volume: 6

Pages Range: 23874-89

Journal Issue: 27

DOI: 10.18632/oncotarget.4381

Abstract

Expression of miR-200c is a molecular switch to determine cellular fate towards a mesenchymal or epithelial phenotype. miR-200c suppresses the early steps of tumor progression by preventing epithelial-mesenchymal transition (EMT) and intravasation of tumor cells. Unraveling the underlying molecular mechanisms might pinpoint to novel therapeutic options. To better understand these mechanisms it is crucial to identify targets of miR-200c. Here, we employ a combination of quantitative proteomic and bioinformatic strategies to identify novel miR-200c targets. We identify and confirm two subunits of the central cellular kinase protein kinase A (PKA), namely PRKAR1A and PRKACB, to be directly regulated by miR-200c. Notably, siRNA-mediated downregulation of both proteins phenocopies the migratory behavior of breast cancer cells after miR-200c overexpression. Patient data from publicly accessible databases supports a miR-200c-PKA axis. Thus, our study identifies the PKA heteroprotein as an important mediator of miR-200c induced repression of migration in breast cancer cells. By bioinformatics, we define a miRNA target cluster consisting of PRKAR1A, PRKAR2B, PRKACB, and COF2, which is targeted by a group of 14 miRNAs.

Authors with CRIS profile

Involved external institutions

How to cite

APA:

Sigloch, F.C., Burk, U.C., Biniossek, M.L., Brabletz, T., & Schilling, O. (2015). miR-200c dampens cancer cell migration via regulation of protein kinase A subunits. Oncotarget, 6(27), 23874-89. https://doi.org/10.18632/oncotarget.4381

MLA:

Sigloch, Florian Christoph, et al. "miR-200c dampens cancer cell migration via regulation of protein kinase A subunits." Oncotarget 6.27 (2015): 23874-89.

BibTeX: Download