Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production
Ippolito GC, Schelonka RL, Zemlin M, Ivanov I, Kobayashi R, Zemlin C, Gartland GL, Nitschke L, Pelkonen J, Fujihashi K, Rajewsky K, Schroeder HW (2006)
Publication Status: Published
Publication Type: Journal article
Publication year: 2006
Journal
Publisher: ROCKEFELLER UNIV PRESS
Book Volume: 203
Pages Range: 1567-1578
Journal Issue: 6
DOI: 10.1084/jem.20052217
Abstract
Tyrosine and glycine constitute 40% of complementarity determining region 3 of the immunoglobulin heavy chain ( CDR-H3), the center of the classic antigen-binding site. To assess the role of D-H RF1-encoded tyrosine and glycine in regulating CDR-H3 content and potentially influencing B cell function, we created mice limited to a single D-H encoding asparagine, histidine, and arginines in RF1. Tyrosine and glycine content in CDR-H3 was halved. Bone marrow and spleen mature B cell and peritoneal cavity B-1 cell numbers were also halved, whereas marginal zone B cell numbers increased. Serum immunoglobulin G subclass levels and antibody titers to T-dependent and T-independent antigens all declined. Thus, violation of the conserved preference for tyrosine and glycine in D-H RF1 alters CDR-H3 content and impairs B cell development and antibody production.
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Germany (DE)How to cite
APA:
Ippolito, G.C., Schelonka, R.L., Zemlin, M., Ivanov, I., Kobayashi, R., Zemlin, C.,... Schroeder, H.W. (2006). Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production. Journal of Experimental Medicine, 203(6), 1567-1578. https://doi.org/10.1084/jem.20052217
MLA:
Ippolito, Gregory C., et al. "Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production." Journal of Experimental Medicine 203.6 (2006): 1567-1578.
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