Brehmer A, Lindig T, Kumar V, Kikins R, Pieper S, Schrödl F, Neuhuber W (2008)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2008
Book Volume: 34
Pages Range: 1-12
Journal Issue: 12
URI: https://www.scopus.com/record/display.uri?eid=2-s2.0-84898881511&origin=inward
We have compared the three-dimensional (3D) morphology of stubby and spiny neurons derived from the human small intestine. After immunohistochemical triple staining for leu-enkephalin (ENK), vasoactive intestinal peptide (VIP) and neuro filament (NF), neurons were selected and scanned based on their immunoreactivity, whether ENK (stubby) or VIP (spiny). For the 3D reconstruction, we focused on confocal data pre-processing with intensity drop correction, non-blind deconvolution, an additional compression procedure in z-direction, and optimizing segmentation reliability. 3D Slicer software enabled a semi-automated segmentation based on an objective threshold (interrater and intrarater reliability, both 0.99). We found that most dendrites of stubby neurons emerged only from the somal circumference, whereas in spiny neurons, they also emerged from the luminal somal surface. In most neurons, the nucleus was positioned abluminally in its soma. The volumes of spiny neurons were significantly larger than those of stubby neurons (total mean of stubbies 806 ± 128 μm, of spinies 2,316 ± 545 μm ), and spiny neurons had more dendrites (26.3 vs. 11.3). The ratios of somal versus dendritic volumes were 1:1.2 in spiny and 1:0.3 in stubby neurons. In conclusion, 3D reconstruction revealed new differences between stubby and spiny neurons and allowed estimations of volumetric data of these neuron populations. © Springer-Verlag 2008.
APA:
Brehmer, A., Lindig, T., Kumar, V., Kikins, R., Pieper, S., Schrödl, F., & Neuhuber, W. (2008). Spiny versus stubby: 3D reconstruction of human myenteric (type I) neurons. Intensive Care Medicine, 34(12), 1-12.
MLA:
Brehmer, Axel, et al. "Spiny versus stubby: 3D reconstruction of human myenteric (type I) neurons." Intensive Care Medicine 34.12 (2008): 1-12.
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