Brehmer A, Lindig T, Schrödl F, Neuhuber W, Ditterich D, Rexer M, Rupprecht H (2005)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2005
Publisher: Springer Verlag (Germany)
Book Volume: 123
Pages Range: 131-138
Journal Issue: 2
DOI: 10.1007/s00418-005-0757-6
The aim of this study was the morphological and further chemical characterisation of neurons immunoreactive for leu-enkephalin (leuENK). Ten wholemounts of small and large intestinal segments from nine patients were immunohistochemically triple-stained for leuENK/neurofilament 200 (NF)/substance P (SP). Based on their simultaneous NF-reactivity and 3D reconstruction of single NF-reactive cells, 97.5% of leuENK-positive neurons displayed the appearance of stubby neurons: small somata; short, stubby dendrites and one axon. Of these leuENK-reactive stubby neurons, 91.3% did not display co-reactivity for SP whereas 8.7% were SP-co-reactive. As to their axonal projection pattern, 50.4% of the recorded leuENK stubby neurons had axons running orally whereas in 29.4% they ran anally; the directions of the remaining 20.2% could not be determined. No axons were seen to enter into secondary strands of the myenteric plexus. Somal area measurements revealed clearly smaller somata of leuENK-reactive stubby neurons (between 259±47 μm and 487±113 μm) than those of putative sensory type II neurons (between 700±217 μm and 1,164±396 μm). The ratio dendritic field area per somal area of leuENK-reactive stubby neurons was between 2.0 and 2.8 reflecting their short dendrites. Additionally, we estimated the proportion of leuENK-positive neurons in comparison to the putative whole myenteric neuron population in four leuENK/anti-Hu doublestained wholemounts. This proportion ranged between 5.9% and 8.3%. We suggest leuENK-reactive stubby neurons to be muscle motor neurons and/or ascending interneurons. Furthermore, we explain why we do not use the term "Dogiel type I neurons" for this population. © Springer-Verlag 2005.
APA:
Brehmer, A., Lindig, T., Schrödl, F., Neuhuber, W., Ditterich, D., Rexer, M., & Rupprecht, H. (2005). Morphology of enkephalin-immunoreactive myenteric neurons in the human gut. Histochemistry and Cell Biology, 123(2), 131-138. https://doi.org/10.1007/s00418-005-0757-6
MLA:
Brehmer, Axel, et al. "Morphology of enkephalin-immunoreactive myenteric neurons in the human gut." Histochemistry and Cell Biology 123.2 (2005): 131-138.
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