Stein MF, Lang S, Winkler T, Deinzer A, Erber S, Nettelbeck DM, Naschberger E, Jochmann R, Stürzl M, Slany R, Werner T, Steinkasserer A, Knippertz I (2013)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2013
Book Volume: 33
Pages Range: 1331-1344
Journal Issue: 7
DOI: 10.1128/MCB.01051-12
CD83 is one of the best-known surface markers for fully mature dendritic cells (mature DCs), and its cell-type-and maturationspecific regulation makes the CD83 promoter an interesting tool for the genetic modulation of DCs. To determine the mechanisms regulating this DC-and maturation-specific CD83 expression, chromatin immunoprecipitation (ChIP)-on-chip microarray, biocomputational, reporter, electrophoretic mobility shift assay (EMSA), and ChIP analyses were performed. These studies led to the identification of a ternary transcriptional activation complex composed of an upstream regulatory element, a minimal promoter, and an enhancer, which have not been reported in this arrangement for any other gene so far. Notably, these DNA regions contain a complex framework of interferon regulatory factor (IRF)-and NF-κB transcription factor-binding sites mediating their arrangement. Mutation of any of the IRF-binding sites resulted in a significant loss of promoter activity, whereas overexpression of NF-κB transcription factors clearly enhanced transcription. We identified IRF-1, IRF-2, IRF-5, p50, p65, and cRel to be involved in regulating maturation-specific CD83 expression in DCs. Therefore, the characterization of this promoter complex not only contributes to the knowledge of DC-specific gene regulation but also suggests the involvement of a transcriptional module with binding sites separated into distinct regions in transcriptional activation as well as cell-type-and maturation-specific transcriptional targeting of DCs. © 2013, American Society for Microbiology.
APA:
Stein, M.F., Lang, S., Winkler, T., Deinzer, A., Erber, S., Nettelbeck, D.M.,... Knippertz, I. (2013). Multiple interferon regulatory factor and NF-κB sites cooperate in mediating cell-type-and maturation-specific activation of the human CD83 promoter in dendritic cells. Molecular and Cellular Biology, 33(7), 1331-1344. https://doi.org/10.1128/MCB.01051-12
MLA:
Stein, Marcello F., et al. "Multiple interferon regulatory factor and NF-κB sites cooperate in mediating cell-type-and maturation-specific activation of the human CD83 promoter in dendritic cells." Molecular and Cellular Biology 33.7 (2013): 1331-1344.
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