Crybb2 coding for ?B2-crystallin affects sensorimotor gating and hippocampal function
Sun M, Hoelter SM, Stepan J, Garrett L, Genius J, Kremmer E, De Angelis MH, Wurst W, Lie DC, Bally-Cuif L, Eder M, Rujescu D, Graw J (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 24
Pages Range: 333-48
Journal Issue: 9-10
DOI: 10.1007/s00335-013-9478-7
Abstract
?B2-crystallin (gene symbol: Crybb2/CRYBB2) was first described as a structural protein of the ocular lens. This gene, however, is also expressed in several regions of the mammalian brain, although its function in this organ remains entirely unknown. To unravel some aspects of its function in the brain, we combined behavioral, neuroanatomical, and physiological analyses in a novel Crybb2 mouse mutant, O377. Behavioral tests with male O377 mutants revealed altered sensorimotor gating, suggesting modified neuronal functions. Since these mouse mutants also displayed reduced hippocampal size, we concentrated further investigations on the hippocampus. Free intracellular Ca(2+) levels were increased and apoptosis was enhanced in the hippocampus of O377 mutants. Moreover, the expression of the gene encoding calpain 3 (gene symbol Capn3) was elevated and the expression of genes coding for the NMDA receptor subunits was downregulated. Additionally, the number of parvalbumin-positive interneurons was decreased in the hippocampus but not in the cortex of the mutants. High-speed voltage-sensitive dye imaging demonstrated an increased translation of input-to-output neuronal activity in the dentate gyrus of this Crybb2 mutant. These results point to an important function of ?B2-crystallin in the hippocampal network. They indicate pleiotropic effects of mutations in the Crybb2 gene, which previously had been considered to be specific to the ocular lens. Moreover, our results are the first to demonstrate that ?B2-crystallin has a role in hippocampal function and behavioral phenotypes. This model can now be further explored by future experiments.
Authors with CRIS profile
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Max-Planck-Institut für Psychiatrie (Deutsche Forschungsanstalt für Psychiatrie)
Germany (DE)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Germany (DE)
Max-Planck-Institut für Psychiatrie (Deutsche Forschungsanstalt für Psychiatrie)
Germany (DE)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
How to cite
APA:
Sun, M., Hoelter, S.M., Stepan, J., Garrett, L., Genius, J., Kremmer, E.,... Graw, J. (2013). Crybb2 coding for ?B2-crystallin affects sensorimotor gating and hippocampal function. Mammalian Genome, 24(9-10), 333-48. https://doi.org/10.1007/s00335-013-9478-7
MLA:
Sun, Minxuan, et al. "Crybb2 coding for ?B2-crystallin affects sensorimotor gating and hippocampal function." Mammalian Genome 24.9-10 (2013): 333-48.
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