Niebler S, Angele P, Kujat R, Boßerhoff AK (2015)
Publication Type: Journal article
Publication year: 2015
Publisher: Hindawi Publishing Corporation
Book Volume: 2015
Pages Range: 380590
DOI: 10.1155/2015/380590
The transcription factor AP-2? (activating enhancer-binding protein epsilon) is expressed in cartilage of humans and mice. However, knowledge about regulatory mechanisms influencing AP-2? expression is limited. Using quantitative real time PCR, we detected a significant increase in AP-2? mRNA expression comparing initial and late stages of chondrogenic differentiation processes in vitro and in vivo. Interestingly, in these samples the expression pattern of the prominent hypoxia marker gene angiopoietin-like 4 (Angptl4) strongly correlated with that of AP-2? suggesting that hypoxia might represent an external regulator of AP-2? expression in mammals. In order to show this, experiments directly targeting the activity of hypoxia-inducible factor-1 (HIF1), the complex mediating responses to oxygen deprivation, were performed. While the HIF1-activating compounds 2,2'-dipyridyl and desferrioxamine resulted in significantly enhanced mRNA concentration of AP-2?, siRNA against HIF1? led to a significantly reduced expression rate of AP-2?. Additionally, we detected a significant upregulation of the AP-2? mRNA level after oxygen deprivation. In sum, these different experimental approaches revealed a novel role for the HIF1 complex in the regulation of the AP-2? gene in cartilaginous cells and underlined the important role of hypoxia as an important external regulatory stimulus during chondrogenic differentiation modulating the expression of downstream transcription factors.
APA:
Niebler, S., Angele, P., Kujat, R., & Boßerhoff, A.K. (2015). Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation. BioMed Research International, 2015, 380590. https://doi.org/10.1155/2015/380590
MLA:
Niebler, Stephan, et al. "Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation." BioMed Research International 2015 (2015): 380590.
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