Time-dependent morphological alterations and viability of cultured human trabecular cells after exposure to Trypan blue
Tsaousis K, Kopsachilis N, Tsinopoulos IT, Dimitrakos SA, Kruse F, Welge-Luessen U (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 41
Pages Range: 484-90
Journal Issue: 5
DOI: 10.1111/ceo.12018
Abstract
To determine the time-dependent toxicity of Trypan blue at 0.06% concentration in cultured human trabecular meshwork cells.Human trabecular meshwork cells cultured in vitro were exposed to Trypan blue and acute toxicity was evaluated. Cells were exposed for 5, 30, 60 s and for 5, 15, 30, 60 min to a commercially available Trypan blue preparation (Vision Blue; DORC International Zuidland, The Netherlands). Morphology was observed by phase-contrast microscopy and cell viability was measured using Hoechst 33342 (Intergen; Purchase, NY, USA) and propidium iodide assays to determine the percentage of living and dead cells.Morphological changes occurred mainly after 5 min of exposure to Trypan blue. Viability was 96.0% ± 3.6%, 94.8% ± 3.5% and 92.5% ± 4.4% after 5, 30 and 60 s of exposure, respectively; a significant toxic effect of Trypan blue was observed after 5, 15, 30 and 60 min of exposure with a viability of 85.0% ± 3.7%, 77.0% ± 6.7%, 70.8% ± 5.9% and 68.3% ± 8.7%, respectively.Trypan blue is not toxic, in terms of cell viability, over an exposure time of up to 60 s; however, further exposure results in a gradual increase in damage of cultured human trabecular meshwork cells.
Authors with CRIS profile
Konstantinos Tsaousis
Professur für Experimentelle Augenheilkunde
Friedrich Kruse
Lehrstuhl für Augenheilkunde
Involved external institutions
Greece (GR)How to cite
APA:
Tsaousis, K., Kopsachilis, N., Tsinopoulos, I.T., Dimitrakos, S.A., Kruse, F., & Welge-Luessen, U. (2013). Time-dependent morphological alterations and viability of cultured human trabecular cells after exposure to Trypan blue. Clinical and Experimental Ophthalmology, 41(5), 484-90. https://doi.org/10.1111/ceo.12018
MLA:
Tsaousis, Konstantinos, et al. "Time-dependent morphological alterations and viability of cultured human trabecular cells after exposure to Trypan blue." Clinical and Experimental Ophthalmology 41.5 (2013): 484-90.
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