Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages - An immunohistochemical analysis

Weber M, Büttner M, Hyckel P, Moebius P, Distel L, Ries J, Amann KU, Neukam FW, Wehrhan F (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Journal of Cranio-Maxillofacial Surgery Elsevier

Publisher: Elsevier

Book Volume: 42

Pages Range: 1087-94

Journal Issue: 7

DOI: 10.1016/j.jcms.2014.01.035

Abstract

In solid malignancies the influence of immunological parameters - especially of macrophages - on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters.T1 and T2 oscc samples (n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively.Primary tumours with lymphogenic metastasis (N+) showed a significantly (p < 0.05) increased count of CD68, CD11c, CD163 and MRC1 positive cells in the epithelial fraction compared to N0 tumours. The ratio of CD163 positive cells (M2 macrophages) to CD68 positive cells (M1 and M2 macrophages) was significantly (p < 0.05) increased in N+ tumours.An increased macrophage infiltration and an increased M2 polarization in primary oral squamous cell carcinomas with lymphogenic metastasis was shown. Macrophages that migrated into the epithelial tumour fraction seem to be of special biological importance. The results indicate a central role of macrophages in the progression of oscc.

Authors with CRIS profile

Manuel Weber Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie Luitpold Distel Department of Radiation Oncology Kerstin Ute Amann Department of Nephropathology Friedrich Wilhelm Neukam Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie Falk Wehrhan Department of Oral and Cranio-Maxillofacial Surgery

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE)

How to cite

APA:

Weber, M., Büttner, M., Hyckel, P., Moebius, P., Distel, L., Ries, J.,... Wehrhan, F. (2014). Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages - An immunohistochemical analysis. Journal of Cranio-Maxillofacial Surgery, 42(7), 1087-94. https://doi.org/10.1016/j.jcms.2014.01.035

MLA:

Weber, Manuel, et al. "Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages - An immunohistochemical analysis." Journal of Cranio-Maxillofacial Surgery 42.7 (2014): 1087-94.

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